| Autor: |
Suresh, P., Johnson Rajeswar, J., Sukumar, K., Harikrishnan, T. J., Srinivasan, P. |
| Předmět: |
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| Zdroj: |
British Poultry Science; Apr2017, Vol. 58 Issue 2, p111-115, 5p |
| Abstrakt: |
1. A study was undertaken to characterise the oncogeneMeqat the molecular level for three serotype 1 Marek’s disease virus (MDV) field isolates from vaccinated poultry flocks which had encountered a Marek’s disease outbreak in the southern part of India. The isolates were named Ind/TN/11/01, Ind/KA/12/02 and Ind/TN/12/03. The oncogeneMeqwas amplified by PCR and sequenced. 2. The isolates were shown to have a homology for theMeqgene of 99.1–99.8% with various isolates from China and 98.5–99.2% with isolates from Europe and the USA. Alignment analysis of the nucleotide sequences showed that nucleotide mutations at 5 different positions in theMeqgene displayed perfect regularity in MDVs circulating in the southern part of India, which could be considered as features of field MDVs recently prevalent in this area. 3. In addition, the mutation in theMeqgene at positions 251, 260 and 437 was unique and coincides with very virulent strains from China GX0101, GXY2 and a Hungarian strain ATE. The mutation at positions 283 and 300 was unique and coincides with the very virulent strain ATE of Hungary. There were also single nucleotide mutations at positions 155 (A–T), 369 (A–C), 462 (C–A) and 548 (C–T) observed in the isolate Ind/TN/12/03. 4. Phylogenetic analysis ofMeqsequences revealed that field MDVs in this area evolved independently but have similarities with very virulent strains from China, and thatMeqhas more similarities with the very virulent Hungarian strain. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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