Exploration of anti-cancer effects and mechanisms of Zuo-Jin-Wan and its alkaloid components in vitro and in orthotopic HepG2 xenograft immunocompetent mice.

Autor:	Shun-Ting Chou, Chien-Yun Hsiang, Hsin-Yi Lo, Hui-Fen Huang, Ming-Tsung Lai, Ching-Liang Hsieh, Su-Yin Chiang, Tin-Yun Ho
Předmět:	ALKALOIDS ANIMAL experimentation ANTINEOPLASTIC agents CELL lines HEPATOCELLULAR carcinoma HERBAL medicine HISTOLOGY LIVER tumors CHINESE medicine MICE PHYSICAL diagnosis POLYMERASE chain reaction RESEARCH funding XENOGRAFTS PLANT extracts NEOPLASTIC cell transformation IN vitro studies ONE-way analysis of variance IN vivo studies
Zdroj:	BMC Complementary & Alternative Medicine; 2/20/2017, Vol. 17, p1-11, 11p, 1 Diagram, 1 Chart, 4 Graphs
Abstrakt:	Background: Zuo-Jin-Wan (ZJW), a two-herb formula consisting of Coptis chinensis (CC) and Evodia rutaecarpa (ER), is commonly used in traditional Chinese medicine for the treatment of cancers. However, the efficacies and mechanisms of ZJW and its alkaloid components on cancers are still unclear. Methods: Here we investigated the anti-cancer effects and mechanisms of ZJW, CC, ER, berberine, and evodiamine in cells and in intrahepatic xenograft mice. Results: Treatment of HepG2 cells with ZJW, CC, ER, berberine, and evodiamine significantly displayed cytotoxic effects in a dose- and time-dependent manner. Hierarchical cluster analysis of gene expression profiles showed that CC and ZJW shared a similar mechanism for the cytotoxic effects, suggesting that CC was the active ingredient of ZJW for anti-cancer activity. Network analysis further showed that c-myc was the likely key molecule involved in the regulation of ZJW-affected gene expression. A human hepatoma xenograft model was established by intrahepatic injection of HepG2 cells containing nuclear factor-?B-driven luciferase genes in immunocompetent mice. In vivo bioluminescence imaging showed that cells had been successfully transplanted in mouse liver. Oral administration of ZJW for 28 consecutive days led to a significant decrease in the accumulation of ascites, the ratio of tumor-to-liver, and the number of transplanted cells in livers. Conclusions: In conclusion, our findings suggested for the first time that ZJW significantly suppressed human cancer cell growth in orthotopic HepG2 xenograft-bearing immunocompetent mice. Moreover, c-myc might play a potent role in the cytotoxic mechanisms of ZJW, CC, ER, berberine, and evodiamine. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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