Anthranilic acid: a free amino-acid pheromone in the black chafer, Holotrichia loochooana loochooana.

Autor: Hiroe Yasui, Sadao Wakamura, Norio Arakaki, Mitsunobu Kishita, Yasutsune Sadoyama
Předmět:
Zdroj: Chemoecology; Jun2003, Vol. 13 Issue 2, p75-80, 6p
Abstrakt: Summary. Ether extract of the abdominal gland of female blackchafers Holotrichia loochooana loochooana (Sawada) (Coleoptera:Scarabaeidae) induced a series of pre-mating behaviors that includedshort-distance orientation, mounting and abdominal bending towardfemales in the laboratory. When the extract was chromatographed on asilica gel column, the activity was eluted with 50% ether in hexane andether fractions. GC-MS analyses revealed that both the active fractionscontained anthranilic acid (2-aminobenzoic acid) as a major compound.When partitioned to basic, acidic and amphoteric fractions, pheromonalactivity was observed with the latter two fractions and anthranilic acidwas detected in these fractions by HPLC analyses. The amount ofanthranilic acid in the female extract was estimated to be ca. 1.3μg/female. Authentic anthranilic acid induced pre-mating behavior inmales of at levels equal to that elicited by the compound in theextract. It also attracted male beetles to the traps in the field. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index