Cu/Zn superoxide dismutase in yeast mitochondria – a general phenomenon

Autor: Nedeva, Trayana S., Petrova, Ventzislava Y., Zamfirova, Daniela R., Stephanova, Elena V., Kujumdzieva, Anna V.
Předmět:
Zdroj: FEMS Microbiology Letters; Jan2004, Vol. 230 Issue 1, p19, 7p
Abstrakt: Fermentative and respiratory yeast strains of genera Saccharomyces, Kluyveromyces, Pichia, Candida and Hansenula have been investigated for mitochondrial localization of Cu/Zn superoxide dismutase (SOD). Pure mitochondrial fractions were obtained and the specific activities of Cu/Zn and Mn SODs were measured in comparison with those in the corresponding cell-free extracts. The Cu/Zn SOD: Mn SOD ratio in mitochondria and crude extracts was calculated and was considered a specific characteristic of all tested strains. Electrophoretical visualization of SOD patterns provided evidence for possible migration of cytosolic Cu/Zn SOD to mitochondria. The characteristic Cu/Zn SOD profile in mitochondria of all tested strains suggested its ubiquity within the fermentative and respiratory yeasts. [Copyright &y& Elsevier]
Databáze: Complementary Index