| Autor: |
Yuzbashev, Tigran V., Bondarenko, Pavel Yu., Sobolevskaya, Tatiana I., Yuzbasheva, Evgeniya Yu., Laptev, Ivan A., Kachala, Vadim V., Fedorov, Alexander S., Vybornaya, Tatiana V., Larina, Anna S., Sineoky, Sergey P. |
| Zdroj: |
Biotechnology & Bioengineering; Nov2016, Vol. 113 Issue 11, p2425-2432, 8p |
| Abstrakt: |
ABSTRACT Bio-based succinic acid production can redirect industrial chemistry processes from using limited hydrocarbons to renewable carbohydrates. A fermentation process that does not require pH-titrating agents will be advantageous to the industry. Previously, a Yarrowia lipolytica strain that was defective for succinate dehydrogenase was constructed and was found to accumulate up to 17.5 g L−1 of succinic acid when grown on glycerol without buffering. Here, a derivative mutant was isolated that produced 40.5 g L−1 of succinic acid in 36 h with a yield of 0.32 g g−1 glycerol. A combination approach of induced mutagenesis and metabolic evolution allowed isolation of another derivative that could utilize glucose efficiently and accumulated 50.2 g L−1 succinic acid in 54 h with a yield of 0.43 g g−1. The parent strain of these isolated mutants was used for [1,6-13C2]glucose assimilation analysis. At least 35% glucose was estimated to be utilized through the pentose phosphate pathway, while ≥84% succinic acid was formed through the oxidative branch of the tricarboxylic acid cycle. Biotechnol. Bioeng. 2016;113: 2425-2432. © 2016 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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