| Autor: |
Destro, FC, Martin, I, Landim‐Alvarenga, FDC, Ferreira, JCP, Pate, JL |
| Předmět: |
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| Zdroj: |
Reproduction in Domestic Animals; Oct2016, Vol. 51 Issue 5, p848-852, 5p |
| Abstrakt: |
Contents The aim of this study was to evaluate the effects of concanavalin A ( CONA) on the progesterone (P4) production by bovine steroidogenic luteal cells ( LCs) in vitro. Luteal cells were collected during the mid-luteal stage (at 10-12 days following ovulation) and processed in the laboratory. Luteal cells were grown for 7 days in a humid atmosphere with 5% CO2, with or without 10% foetal bovine serum, and were subjected to the following treatments: control: no treatment; CONA (10 μg/ml); LH (100 μg/ml); CONA + LH; LH (100 μg/ml) + prostaglandin F2α ( PGF2α) (10 ng/ml); CONA + LH + PGF2α. Samples of the culture media were collected on days 1 (D1) and 7 (D7) for P4 quantification. The cells were counted on D7 of culture. Differences between treatments were considered statistically significant at p < .05. Culture in the presence of CONA decreased the P4-secreting capacity of LCs on D7 of culture, particularly in the absence of serum. The cell numbers did not change between treatments. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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