| Autor: |
Isabey, Daniel, Pelle, Gabriel, André Dias, Sofia, Bottier, Mathieu, Nguyen, Ngoc-Minh, Filoche, Marcel, Louis, Bruno |
| Předmět: |
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| Zdroj: |
Biomechanics & Modeling in Mechanobiology; Aug2016, Vol. 15 Issue 4, p947-963, 17p |
| Abstrakt: |
Cellular adhesion forces depend on local biological conditions meaning that adhesion characterization must be performed while preserving cellular integrity. We presently postulate that magnetic bead twisting provides an appropriate stress, i.e., basically a clamp, for assessment in living cells of both cellular adhesion and mechanical properties of the cytoskeleton. A global dissociation rate obeying a Bell-type model was used to determine the natural dissociation rate ( $$K_\mathrm{off}^0$$ ) and a reference stress ( $$\sigma _c$$ ). These adhesion parameters were determined in parallel to the mechanical properties for a variety of biological conditions in which either adhesion or cytoskeleton was selectively weakened or strengthened by changing successively ligand concentration, actin polymerization level (by treating with cytochalasin D), level of exerted stress (by increasing magnetic torque), and cell environment (by using rigid and soft 3D matrices). On the whole, this multiscale evaluation of the cellular and molecular responses to a controlled stress reveals an evolution which is consistent with stochastic multiple bond theories and with literature results obtained with other molecular techniques. Present results confirm the validity of the proposed bead-twisting approach for its capability to probe cellular and molecular responses in a variety of biological conditions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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