Cardioprotection provided by Echinatin against ischemia/reperfusion in isolated rat hearts.

Autor:	Xing-han Tian, Chao-liang Liu, Hai-Li Jiang, Yan Zhang, Ji-chun Han, Ju Liu, Meng Chen, Tian, Xing-Han, Liu, Chao-Liang, Jiang, Hai-Li, Zhang, Yan, Han, Ji-Chun, Liu, Ju, Chen, Meng
Předmět:	LACTATE dehydrogenase MYOCARDIAL infarction TETRAZOLIUM chloride CREATINE kinase MALONDIALDEHYDE HEART metabolism ANIMAL experimentation ANTI-inflammatory agents ANTIOXIDANTS APOPTOSIS BLOOD pressure CARDIOTONIC agents CORONARY circulation DOSE-effect relationship in pharmacology FLAVONOIDS LEFT heart ventricle HEART physiology HEART ventricles HEART beat INFLAMMATORY mediators INTERLEUKINS MYOCARDIAL reperfusion complications MYOCARDIUM PHARMACOLOGY RATS SUPEROXIDE dismutase TIME TUMOR necrosis factors OXIDATIVE stress PHARMACODYNAMICS PREVENTION
Zdroj:	BMC Cardiovascular Disorders; 5/31/2016, Vol. 16, p1-9, 9p, 1 Diagram, 6 Graphs
Abstrakt:	Background: This study evaluated the protective effect of Echinatin against myocardial ischemia/reperfusion (I/R) injury in rats.Methods: The effect of Echinatin on cardiac function in rats subjected to I/R was demonstrated through improved Langendorff retrograde perfusion technology. Adult Sprague-Dawley rats were randomly divided into five groups, and myocardial infarct size was macroscopically estimated through 2,3,5-triphenyltetrazolium chloride staining. The coronary effluent was analyzed for the release of lactate dehydrogenase (LDH) and creatine kinase (CK) to assess the degree of cardiac injury. The concentrations of malondialdehyde (MDA), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were determined along with superoxide dismutase (SOD) activity using ELISA. Finally, cardiomyocyte apoptosis analysis was conducted with POD, an in situ cell death detection kit.Results: Echinatin (0.5 and 2.5 μg/mL) pretreatment enhanced the maximum up/down rate of the left ventricular pressure (±dp/dtmax), improved the heart rate, increased the left ventricular developed pressure (LVDP), enhanced the coronary flow, and reduced the CK and LDH levels in the coronary flow of the treated group compared with the I/R group. Echinatin limited the contents of CK and LDH, improved the LVDP, reduced the contents of MDA, IL-6, and TNF-α, and increased the SOD activity. The infarct size and cell apoptosis in the hearts of the rats in the Echinatin-treated group were smaller and lower, respectively, than those in the hearts of the rats in the I/R control group.Conclusion: Echinatin exerts a protective effect against I/R-induced myocardial injury on hearts. This effect may be attributed to the antioxidant and anti-inflammatory activities of this compound. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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