| Autor: |
Winfield, Mark, Ibrahim, A.H., Jones, A., Protheroe, R.K.M., Gunaserkera, J.B.L., Wragg, C.S., Vilain-Holmes, A., Ross, F.M. |
| Předmět: |
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| Zdroj: |
Journal of Medical Genetics; Sep2003 Supplement, Vol. 40, pS23-S23, 1/4p |
| Abstrakt: |
Multiple myeloma (MM) results from the neoplastic proliferation of terminally differentiated plasma cells in the bone marrow. Myeloma karyotypes are usually complex with gains, losses and structural alterations of many chromosomes. Given this chaotic backdrop, we have used FISH analysis to try and identify events that might represent primary lesions or those that have prognostic importance and so may help in the staging of patients and in the choice of targeted treatments. Interphase FISH was carried out on purified plasma cells from 219 cases of MM and 27 cases of monoclonal gammopathy of undetermined significance (MGUS, an asymptomatic plasma cell dyscrasia that may represent a benign precursor of MM). Centromeric probes were used for chromosomes (3, 6, 7, 9, 10, 11, and 17) in order to identify monosomies, trisomies and hypo- and hyperdiploidy. Particular attention was paid to chromosomes 13 (loss of 13 is considered to be an indicator of poor prognosis) and 14 (rearrangements involving the immunoglobulin heavy chain locus on 14q are candidates for a primary lesion). Overall, 95% of cases showed abnormality of at least one of these chromosomes; 37% had partial or complete deletion of chromosome 13; only 43% had visible translocations involving the IgH locus. MGUS had the same spectrum of alterations as MM but always at a lower frequency. An attempt to correlate karyotypic features with a range of diagnostic criteria was also carried out. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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