| Autor: |
Maziero, RRD, Guaitolini, CRF, Paschoal, DM, Kievitsbosch, T, Guastali, MD, Moraes, CN, Landim‐Alvarenga, FC |
| Předmět: |
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| Zdroj: |
Reproduction in Domestic Animals; Apr2016, Vol. 51 Issue 2, p204-211, 8p |
| Abstrakt: |
Contents This study aimed to produce in vitro bovine embryos by the addition of two drugs, which is responsible for oocyte meiosis inhibition: roscovitine ( ROS) and butyrolactone I ( BL-I). Oocytes were recovered from slaughtered cows and matured in a commercial medium and maintained in a 5% CO2 atmosphere. Oocytes were maintained for 6 h in an in vitro maturation ( IVM) medium containing ROS (12.5 μ m), BL-I (50 μ m) and association of drugs ( ROS 6.25 μ m and BL-I 25 μ m). Oocytes were cultured for 18 h in an agent-free medium for the resumption of meiosis. After 24 h of maturation, oocytes were inseminated in the commercial in vitro fertilization ( IVF) medium. Presumptive zygotes were cultured in SOFaa medium in a 5% CO2 atmosphere. On day 3, rate of cleavage was evaluated and on days 6 and 7, rate of blastocyst formation. BL-I and its association with the ROS increased the rates of cleavage and blastocyst formation (p < 0.05). The ROS alone was inefficient, impairing embryonic development, with low rates of blastocyst formation when compared to the control group and other treatments (p < 0.05). The embryos from BL-I and ROS+ BL-I groups presented higher number of cells and lower rates of cellular apoptosis compared to other groups, either for the fresh or for post-thawing embryos. Embryos from ROS+ BL-I group showed to be more resistant to the vitrification process, presenting a higher rate of embryonic re-expansion (p < 0.05). In conclusion, block of meiosis using BL-I or its association with ROS increased the rate of blastocyst formation, and the association of ROS+ BL-I resulted in a better resistance to the embryo cryopreservation process. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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