| Autor: |
Chaitanya, B. N., Asokan, R., Sita, T., Rebijith, K. B., Krishna Kumar, N. K. |
| Předmět: |
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| Zdroj: |
Annals of the Entomological Society of America; Jan2016, Vol. 109 Issue 1, p92-98, 7p |
| Abstrakt: |
Overuse of chemical insecticides such as organophosphates, pyrethroids, and endosulfan in managing notorious sap-sucking insect pests such as the melon aphid, Aphis gossypii Glover, resulted in insecticide resistance. To combat the above and bring out the novel pest control strategy, we implemented ribonucleic acid interference (RNAi), a reverse genetics tool in silencing the target genes. RNAi is a sequence-specific gene-silencing mechanism triggered by double-stranded ribonucleic acid (dsRNA). The efficiency of RNAi is reliant upon concentration of dsRNA, mode of delivery, gene targets, insect species, etc. The objective of the present study was to assess the extent of down-regulation by using two different concentrations of dsRNA specific to sodium channel (AgSCN) and ultraspiracle genes (AgUSP) (0.0625 and 0.125 μg/μl) with two time intervals (48 and 96h) through oral delivery. The extent of declined gene expression and percentage mortality chronicled for sodium channel and USP is proportional to the dsRNA concentration. Thus, our study affirms the outlook in the development of RNAi which can form a potent species-specific tool in the management of insect pests such as A. gossypii. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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