| Autor: |
Alekseeva, O., Gusel'nikova, V., Beznin, G., Korzhevskii, D. |
| Předmět: |
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| Zdroj: |
Journal of Evolutionary Biochemistry & Physiology; Sep2015, Vol. 51 Issue 5, p357-369, 13p |
| Abstrakt: |
Assessment of the integrity, viability and functional state of nerve cells is a major problem in studying the effect of experimental exposures on different brain structures in vertebrates and correlating structural abnormalities with changes in physiological parameters. Such an assessment is possible due to the use of the immunocytochemical reaction for NeuN, a neuronal nuclear protein discovered in 1992. Numerous studies have demonstrated that this protein is neuron-specific, while its amino acid sequence shows a high degree of interspecies conservatism. In the present review, the available information about the functions of NeuN in nerve cells and results of its application for the assessment of the functional state and viability of CNS neurons during experiments are generalized and analyzed. A special focus is on the critical analysis of the data that allowed the properties and functions of NeuN to be deduced from. The still poorly explained facts of the constitutionally lacking expression of this biomarker in some neuronal populations of the mammalian brain and spinal cord are also discussed. The analysis of our own and literature data reveals a great potential of the NeuN labeling in studying the responses of nerve cells to damage, including comparative interspecies studies. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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