| Autor: |
Escalona, E. E., Leng, J., Dona, A. C., Merrifield, C. A., Holmes, E., Proudman, C. J., Swann, J. R. |
| Zdroj: |
Equine Veterinary Journal; Nov2015, Vol. 47 Issue 6, p721-730, 9p |
| Abstrakt: |
Reasons for performing study Metabonomics is emerging as a powerful tool for disease screening and investigating mammalian metabolism. This study aims to create a metabolic framework by producing a preliminary reference guide for the normal equine metabolic milieu. Objectives To metabolically profile plasma, urine and faecal water from healthy racehorses using high resolution 1 H-nuclear magnetic resonance ( NMR) spectroscopy and to provide a list of dominant metabolites present in each biofluid for the benefit of future research in this area. Study design This study was performed using 7 Thoroughbreds in race training at a single time point. Urine and faecal samples were collected noninvasively and plasma was obtained from samples taken for routine clinical chemistry purposes. Methods Biofluids were analysed using 1 H- NMR spectroscopy. Metabolite assignment was achieved via a range of one- and 2-dimensional experiments. Results A total of 102 metabolites were assigned across the 3 biological matrices. A core metabonome of 14 metabolites was ubiquitous across all biofluids. All biological matrices provided a unique window on different aspects of systematic metabolism. Urine was the most populated metabolite matrix with 65 identified metabolites, 39 of which were unique to this biological compartment. A number of these were related to gut microbial host cometabolism. Faecal samples were the most metabolically variable between animals; acetate was responsible for the majority (28%) of this variation. Short-chain fatty acids were the predominant features identified within this biofluid by 1 H- NMR spectroscopy. Conclusions Metabonomics provides a platform for investigating complex and dynamic interactions between the host and its consortium of gut microbes and has the potential to uncover markers for health and disease in a variety of biofluids. Inherent variation in faecal extracts along with the relative abundance of microbial-mammalian metabolites in urine and invasive nature of plasma sampling, infers that urine is the most appropriate biofluid for the purposes of metabonomic analysis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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