| Autor: |
Nishina, Atsuyoshi, Ebina, Kodai, Ukiya, Motohiko, Fukatsu, Makoto, Koketsu, Mamoru, Ninomiya, Masayuki, Sato, Daisuke, Kimura, Hirokazu |
| Předmět: |
|
| Zdroj: |
Journal of Food Science (John Wiley & Sons, Inc.); Oct2015, Vol. 80 Issue 10, pH2354-H2359, 6p, 4 Charts, 1 Graph |
| Abstrakt: |
This study aimed to chemically isolate and explore an antimelanogenesis inducer in extracts of Solanum melongena L. 'Usukawamarunasu' eggplant. We successfully identified dioscin ([25R]-Spirost-5-en-3β-yl) 2- O-(6-deoxy-α- l-mannopyranosyl) - 4- O-(6-deoxy-α- l-mannopyranosyl)-β- d-glucopyranoside] in the plant, and examined the effects of α-melanocyte-stimulating hormone (MSH)-induced melanogenesis in B16 murine melanoma cells by this plant-derived dioscin. Immunoblot analysis suggested that dioscin reduced the expression of tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2, resulting in inhibition of intracellular production of melanin. In addition, dioscin caused reduction of phosphorylated cAMP-responsive element binding protein 1 transcription factors (CREB), which led to a reduction of microphthalmia-related transcription factor (MITF) in α-MSH-stimulated cells, but did not affect phosphorylation of extracellular signal-regulated kinase. Furthermore, dioscin significantly downregulated the expression of tyrosinase, TRP-1, and TRP-2, which led to the reduction of α-MSH-induced melanogenesis in B16 cells. These results suggest that dioscin may decrease the level of MITF via inhibition of phosphorylation of CREB in α-MSH-induced melanogenesis in B16 cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text