Biochemical Responses of Anodized Titanium Implants with a Poly(lactide-co-glycolide)/Bone Morphogenic Protein-2 Submicron Particle Coating. Part 1: An In Vitro Study.

Autor:	Soo-Yeon Yoo, Seong-Kyun Kim, Seong-Joo Heo, Jai-Young Koak, Joo-Hee Lee, Ji-Man Park
Předmět:	RECOMBINANT proteins BONE morphogenetic proteins ANALYSIS of variance ANIMAL experimentation CELL culture CELL differentiation CELL physiology DENTAL implants MICROSCOPY RABBITS RESEARCH funding STATISTICS STEM cells TITANIUM DATA analysis DATA analysis software DESCRIPTIVE statistics IN vitro studies MANN Whitney U Test KRUSKAL-Wallis Test THERAPEUTICS
Zdroj:	International Journal of Oral & Maxillofacial Implants; 2015, Vol. 30 Issue 3, p512-518, 7p, 3 Black and White Photographs, 4 Graphs
Abstrakt:	Purpose: This study was conducted to examine the effects of coating poly(D,L-lactide-co-glycolide) (PLGA)/ recombinant human bone morphogenetic protein-2 (rhBMP-2) submicron particles by electrospray onto titanium (Ti) implants on the proliferation and differentiation capacity of mesenchymal stem cells. In addition, the duration of rhBMP-2 release was investigated. Materials and Methods: Ti disks were fabricated and divided into four groups as follows. Group C (control) was anodized at 300 V, group P was anodized and then coated with 0.2% PLGA, group B1 was anodized and then coated with PLGA/rhBMP-2 submicron particles at 200 ng per disk, and group B2 was anodized and then coated with PLGA/rhBMP-2 submicron particles at 600 ng per disk. The topography of the PLGA/rhBMP-2-coated Ti surfaces was examined, and proliferation assays, alkaline phosphatase activity tests, and rhBMP-2 releasing tests were conducted in vitro. Results: There was no difference in the roughness of control Ti disks and Ti disks coated with PLGA/rhBMP-2 submicron particles by electrospray. The proliferation of mesenchymal stem cells increased over time; at 7 days, cell proliferation on the Ti disks coated with rhBMP-2 was significantly higher than the other groups. Anodized Ti disks coated with PLGA/rhBMP-2 (groups B1 and B2) released rhBMP-2 for approximately 21 days. In accordance with the amount of rhBMP-2 released from the PLGA/rhBMP-2 construct, the differentiation capacity of mesenchymal stem cells on the PLGA/rhBMP-2-coated disks was increased significantly. Conclusion: Submicron PLGA/rhBMP-2 coating on Ti implants by electrospray facilitated cell proliferation and differentiation, which is important for early healing and integration of implants. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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