Bacillus dabaoshanensis sp. nov., a Cr(VI)-tolerant bacterium isolated from heavy-metal-contaminated soil.

Autor: Cui, Xiaowen, Wang, Yueqiang, Liu, Jing, Chang, Ming, Zhao, Yong, Zhou, Shungui, Zhuang, Li
Předmět:
Zdroj: Archives of Microbiology; May2015, Vol. 197 Issue 4, p513-520, 8p
Abstrakt: A Cr(VI)-tolerant, Gram-staining-positive, rod-shaped, endospore-forming and facultative anaerobic bacterium, designated as GSS04, was isolated from a heavy-metal-contaminated soil. Strain GSS04 was Cr(VI)-tolerant with a minimum inhibitory concentration of 600 mg l and was capable of reducing Cr(VI) under both aerobic and anaerobic conditions. Growth occurred with presence of 0-3 % (w/v) NaCl (optimum 1 %), at pH 5.5-10.0 (optimum pH 7.0) and 15-50 °C (optimum 30-37 °C). The main respiratory quinone was MK-7 and the major fatty acids were anteiso-C and iso-C. The DNA G+C content was 41.1 mol%. The predominant polar lipid was diphosphatidylglycerol. Based on 16S rRNA gene sequence similarity, the closest phylogenetic relative was Bacillus shackletonii DSM 18868 (97.6 %). The DNA-DNA hybridization between GSS04 and its closest relatives revealed low relatedness (<70 %). The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain GSS04 represents a novel species of the genus Bacillus, for which the name Bacillus dabaoshanensis sp. nov. is proposed. The type strain is GSS04 (=CCTCC AB 2013260 = KCTC 33191). [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index