| Autor: |
ZHAO Zhen-fang, LIU Wei, ZHENG Li-ping, FENG Yong-jun, WU Cun-xiang |
| Předmět: |
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| Zdroj: |
Chinese Journal of Oil Crop Sciences; 2014, Vol. 36 Issue 6, p713-719, 7p |
| Abstrakt: |
To explore the relationship between GmNMHC5, a member of MADS-box transcription factor family, and the production of phytohormones in the roots of soybean (Glycine max) cv. Zigongdongdou, GmNMHC5 was transferred to soybean plant by Agrobacterium rhizogenes, the positive transgenic hairy root were selected with GUS staining and relative expression level of GmNMHC5. The content of phytohormones in the transgenic and non-transgenic hairy root(CK) were detected using ELISA method at V3 stage. The results indicated that, in comparison with control, gibberellin A3 (GA3) increased 18.5%, and ABA content decreased 19.6%, respectively. These indicated that the overexpression of GmNMHC5 promoted the production of GA3 and suppressed the production of ABA. After treatment with exogenous GA3 (50 μmol/L) and exogenous ABA (50μmol/L), real-time fluorescent quantitative PCR analysis showed relative expression of GmNMHC5 in root were 4.52 and 0.31 times of the control at V3 stage. It was suggested that the promotional effects of exogenous GA3 and suppressive effects of ABA on the expression of GmNMHC5 were both highly significant in the root of soybean. These data revealed an interactive regulation between the expression of GmNMHC5 and the production of phytohormones in soybean. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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