| Autor: |
Hagedoorn PL; Wageningen University and Research Centre, Department of Biomolecular Sciences, The Netherlands. peter.leonhagedoorn@epr.bc.wau.nl, Driessen MC, van den Bosch M, Landa I, Hagen WR |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1998 Dec 04; Vol. 440 (3), pp. 311-4. |
| DOI: |
10.1016/s0014-5793(98)01466-5 |
| Abstrakt: |
The redox chemistry of Pyrococcus furiosus rubredoxin and ferredoxin has been studied as a function of temperature in direct voltammetry and in EPR monitored bulk titrations. The Ems of both proteins, measured with direct voltammetry, have a normal (linear) temperature dependence and show no pH dependence. EPR monitoring is not a reliable method to determine the temperature dependence of the Em: upon rapid freezing the proteins take their conformation corresponding to the freezing point of the solution. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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