| Autor: |
Wedekind JE; Department of Structural Biology, Stanford University School of Medicine, California 94305-5126, USA. wedekind@ribose.stanford.edu, McKay DB |
| Jazyk: |
angličtina |
| Zdroj: |
Annual review of biophysics and biomolecular structure [Annu Rev Biophys Biomol Struct] 1998; Vol. 27, pp. 475-502. |
| DOI: |
10.1146/annurev.biophys.27.1.475 |
| Abstrakt: |
The hammerhead ribozyme is a small catalytic RNA that cleaves a target phosphodiester bond in a reaction dependent on divalent metal ions. Crystal structures of the hammerhead reveal the tertiary fold of an enzymatic "ground state" of the molecule; however, they do not clarify the catalytic mechanism of the ribozyme, presumably because a significant conformational rearrangement is required to reach an enzymatic transition state. The structural domains seen in the hammerhead can be related to sequence or structural motifs in transfer and ribosomal RNAs, suggesting that they represent tertiary building blocks that will be found in large, complex RNAs. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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