| Autor: |
Manouilov KK; UNMC/Eppley Cancer Center Core Pharmacokinetics Laboratory, College of Pharmacy, University of Nebraska Medical Center, Omaha 68198-6025, USA., McGuire TR, Gordon BG, Gwilt PR |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. B, Biomedical sciences and applications [J Chromatogr B Biomed Sci Appl] 1998 Apr 10; Vol. 707 (1-2), pp. 342-6. |
| DOI: |
10.1016/s0378-4347(97)00543-4 |
| Abstrakt: |
An HPLC assay for etoposide in human serum was developed. Serum, spiked with podophyllotoxin (internal standard), was treated with sodium dodecyl sulphate prior to solid phase extraction. Analysis was performed on a 300x3.9 mm Bondclone 10 C18 column coupled with a fluorometric detector (lambda(ex) 230 nm, lambda(em) 330 nm). The retention times for etoposide and podophyllotoxin were 14 and 28 min respectively. The range of assay was 0.5 to 20 microg/ml with a detection limit of 0.2 microg/ml. This assay is suitable for use in clinical studies with etoposide. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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