HIV-1 and HIV-2 dual infection: lack of HIV-2 provirus correlates with low CD4+ lymphocyte counts.
| Autor: | Sarr AD; Harvard AIDS Institute, Harvard School of Public Health, Boston, Massachusetts 02115, USA., Hamel DJ, Thior I, Kokkotou E, Sankalé JL, Marlink RG, Coll-Seck EM, Essex ME, Siby T, NDoye I, Mboup S, Kanki PJ |
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| Jazyk: | angličtina |
| Zdroj: | AIDS (London, England) [AIDS] 1998 Jan 22; Vol. 12 (2), pp. 131-7. |
| DOI: | 10.1097/00002030-199802000-00002 |
| Abstrakt: | Objective: We conducted this study to genetically characterize dual infection in individuals demonstrating a dual serological profile. Methods: All subjects were first evaluated by immunoblot for antibody reactivity to the major viral antigens for HIV-1 and HIV-2. Sera were judged to be dual-seropositive if they reacted with strong and equal intensity with the envelope antigens of both HIV-1 and HIV-2 and were confirmed with type-specific recombinant env peptides. We used nested polymerase chain reaction (PCR) to amplify proviral gag and env sequence from peripheral blood mononuclear cell (PBMC) DNA from HIV-1- and HIV-2-infected individuals. Positive amplification was detected after Southern blot hybridization. Results: Plasmid dilution and mixing showed equivalent sensitivity of HIV-1 and HIV-2 primers that was not altered by heterologous target sequences. The DNA PCR showed 100% sensitivity and specificity for detection of monotypic HIV infection. Serologically defined HIV-dual reactives were evaluated by this assay, with 100% detection in female sex workers (21 out of 21), but only 38.5% detection (five out of 13) in hospitalized patients; all being HIV-1 positive only. The lack of HIV-2 proviral signal was significantly correlated with low CD4+ lymphocyte counts (Pvalue = 0.04). Conclusion: The results suggest that HIV dual infection may not be a static condition. Levels of HIV-2 may decrease with disease progression or sequester in tissue reservoirs; our results may also suggest that HIV-1 effectively overgrows HIV-2 in the dually exposed host individual. |
| Databáze: | MEDLINE |
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