Determining light dose for photodynamic therapy of atherosclerotic lesions in the Yucatan miniswine.
| Autor: | Hsiang YN; Department of Surgery, University of British Columbia, Vancouver, Canada., Todd ME, Bower RD |
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| Jazyk: | angličtina |
| Zdroj: | Journal of endovascular surgery : the official journal of the International Society for Endovascular Surgery [J Endovasc Surg] 1995 Nov; Vol. 2 (4), pp. 365-71. |
| DOI: | 10.1583/1074-6218(1995)002<0365:DLDFPT>2.0.CO;2 |
| Abstrakt: | Purpose: To determine the light dose required for photodynamic therapy of atherosclerotic lesions in the miniswine. Methods: Aortic atherosclerosis was created in seven Yucatan miniswine by a combination of balloon endothelial injury and 2% cholesterol and 15% lard for 7 weeks. Six animals received the photosensitizer Photofrin 2.5 mg/kg, while an additional swine received no drug. After 24 hours, the abdominal aorta was exposed and the aorta opened longitudinally in each animal. Three 1-cm spots were illuminated with energy densities of 60, 120, and 240 J/cm2 from an argon-pumped dye laser tuned to 630 nm with a laser output of 1 W. Four weeks later, the animals were killed, abdominal aortae removed, and intimal thickness determined by morphometry. Results: The percentage intimal thickness (mean +/- SD) was 36.7 +/- 27.1, 9.1 +/- 5.0, and 6.4 +/- 8.1 for the three energy densities, respectively. Although both 120 and 240 J/cm2 energy densities produced significant (p < 0.05) reduction in atheroma, considerable damage to the underlying media was also observed in the 240 J/cm2 group. Conclusions: A Photofrin dose of 2.5 mg/kg and 120 J/cm2 light are necessary for adequate ablation of atheroma while avoiding extensive medial damage. |
| Databáze: | MEDLINE |
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