| Autor: |
Robinson KA; Andreas Gruentzig Cardiovascular Center, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA., Chronos NA, Schieffer E, Palmer SJ, Cipolla GD, Milner PG, King SB 3rd |
| Jazyk: |
angličtina |
| Zdroj: |
Catheterization and cardiovascular diagnosis [Cathet Cardiovasc Diagn] 1997 Jul; Vol. 41 (3), pp. 348-53. |
| DOI: |
10.1002/(sici)1097-0304(199707)41:3<348::aid-ccd17>3.0.co;2-j |
| Abstrakt: |
Localized delivery of antisense oligonucleotides directed against cell cycle regulatory proteins has been proposed as a means to prevent restenosis after angioplasty. To test whether single endoluminal delivery of a combination of proliferating cell nuclear antigen (PCNA) and cell-division cycle 2 kinase (cdc2) antisense might affect restenosis, we delivered 2 ml of lipid-complexed PCNA/cdc2 antisense oligomers (1.35 mg) to the coronary arteries of pigs after balloon overstretch angioplasty (AS group) and performed planimetric histomorphometry on arterial sections of the tissue, harvested at 4 wk. Compared with controls receiving 3'-5' reversed sequence oligomers (REV group), there were no differences in absolute intimal area (AS 1.36 +/- 0.08 mm2, REV 1.23 +/- 0.10 mm2, P = NS), intimal area normalized to extent of injury (AS 0.67 +/- 0.03, REV 0.77 +/- 0.10, P = NS), or vessel perimeter (AS 7.72 +/- 0.19 mm, REV 7.36 +/- 0.22 mm, P = NS). We conclude that single endoluminal delivery of antisense against key cell cycle regulatory proteins does not affect neointima formation or vessel size in this model of restenosis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Plný text