Abstrakt: |
Electrophoretic analysis showed that the somatic, excretory and secretory, and egg antigens of O. felineus have a complex protein composition. They were found to have 20, 11, and 25 proteins, respectively. On titration of the antigens, by using a constant dose of immune sera, the excretory and secretory antigen was detected in concentrations 50-100 less than the somatic and egg antigens. Rabbit immune sera effectively interacted with the proteins 116, 105, 80, 70, 60, 50, 44, 40 kD as part of the excretory and secretory antigen, with the proteins 105, 80, 70, 60, 50, 44, 40 and 32 kD as part of the somatic antigen, with the proteins p70, p60, p50, p28, p25 and p24 kD as part of the egg one. The limited human immune responses revealed by the author on O. felineus invasion, which was associated with the excretory and secretory and somatic p105 and three egg antigen proteins, such as p74, p70, p64 clearly indicate further ways of improving the immune diagnosis of opisthorchiasis. |