| Autor: |
McCoy JP Jr; Division of Pediatric Hematology/Oncology, Cooper Hospital/University Medical Center, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Camden 08103, USA., Overton WR, Schroeder K, Blumstein L, Donaldson MH |
| Jazyk: |
angličtina |
| Zdroj: |
Cytometry [Cytometry] 1996 Jun 15; Vol. 26 (2), pp. 148-53. |
| DOI: |
10.1002/(SICI)1097-0320(19960615)26:2<148::AID-CYTO8>3.0.CO;2-C |
| Abstrakt: |
The antigenic specificity of the majority of T lymphocytes in the peripheral blood is determined by the combination of alpha and beta variable region chains present in the T cell receptor complex. Currently, the V beta chains are grouped into 25 families. Historically, determination of V beta usage has relied on detection of gene rearrangement on the nucleic acid level; however, with the increased availability of monoclonal antibodies to the product of these genomic rearrangements, immunophenotypic methods are rapidly becoming a reliable alternative method for studying the usage of V beta regions by T cells and T cell subsets. In the present study, multiparametric flow cytometry was used to determine the use of 10 V beta chains on CD4+ and CD8+ T cells in the peripheral blood of 28 normal donors. By obtaining absolute lymphocyte counts at the time blood was drawn, the absolute number of both CD4+ and CD8+ cells using particular V beta regions could be determined. Additionally, the intradonor consistency of V beta usage was examined by obtaining blood from 5 of the volunteers at an interval of approximately 1 year. The results of this study suggest a fairly homogeneous pattern of use for these V beta regions. The most striking longitudinal differences were observed in one individual who underwent a tonsillectomy midway between the T cell receptor V beta determinations. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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