| Autor: |
Berson AE; Applied Immune Sciences, Inc., Santa Clara, CA 95054-1114 USA. lebkowsk@aisx.com, Knobel KM, Rood D, Chen K, Lamons D, McNally MA, Okarma TB, Lebkowski JS |
| Jazyk: |
angličtina |
| Zdroj: |
BioTechniques [Biotechniques] 1996 Jun; Vol. 20 (6), pp. 1098-103. |
| DOI: |
10.2144/96206pf02 |
| Abstrakt: |
We have established rapid procedures that negatively deplete and positively select for specific murine cell populations. By using polystyrene tissue culture flasks containing a covalently bound mouse anti-rat antibody and specific anti-mouse, cell-surface antigen antibodies, we easily and efficiently depleted greater than 90% of the mature lineage cells from murine bone marrow. This selection procedure resulted in an enrichment of progenitor colonies (CFU-Cs) in murine bone marrow. Using the same polystyrene tissue culture devices, we can directly isolate CD117+ (c-kit+) murine hematopoietic cells. As few as 2000 of these CD117+ cells rescued and reconstituted lethally irradiated recipients in a murine bone marrow transplant model. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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