| Autor: |
Liarakos CD; Department of Biochemistry, University of Tennessee, Memphis 38163., Theus SA, Watson AS, Wahba AJ, Dholakia JN |
| Jazyk: |
angličtina |
| Zdroj: |
Archives of biochemistry and biophysics [Arch Biochem Biophys] 1994 Nov 15; Vol. 315 (1), pp. 54-9. |
| DOI: |
10.1006/abbi.1994.1470 |
| Abstrakt: |
We have investigated the role in translation of the secondary structure in the noncoding leader (NCL) sequence of ovalbumin mRNA. Deletion of a stem-loop structure from the mRNA 5'-end (hairpin-1) produced a 2.5-fold decrease in mRNA translation rate in both rabbit reticulocyte wheat germ cell-free systems. A corresponding 2-fold reduction in mRNA binding affinity for reticulocyte eucaryotic initiation factor 2 (eIF-2) was also observed. These effects were independent of mRNA capping. Both translation rate and eIF-2 binding affinity were restored by addition to the mRNA of a sequence containing a hairpin-1-like structure. The positive correlation between cell free translation rate and mRNA binding to eIF-2 suggests that hairpin-1 is both an initiation signal and part of a specific eIF-2 recognition site. Methylation interference indicated a direct interaction between eIF-2 and hairpin-1 of ovalbumin mRNA. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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