| Autor: |
Hartland RP; Department of Plant Biology, University of Groningen, Haren, The Netherlands., Vermeulen CA, Klis FM, Sietsma JH, Wessels JG |
| Jazyk: |
angličtina |
| Zdroj: |
Yeast (Chichester, England) [Yeast] 1994 Dec; Vol. 10 (12), pp. 1591-9. |
| DOI: |
10.1002/yea.320101208 |
| Abstrakt: |
Pulse-chase experiments with [14C]glucose demonstrated that in the cell wall of wild-type Saccharomyces cerevisiae alkali-soluble (1-3)-beta-glucan serves as a precursor for alkali-insoluble (1-3)-beta-glucan. The following observations support the notion that the insolubilization of the glucan is caused by linkage to chitin: (i) degradation of chitin by chitinase completely dissolved the glucan, and (ii) disruption of the gene for chitin synthase 3 prevented the formation of alkali-insoluble glucan. These cells, unable to form a glucan-chitin complex, were highly vulnerable to hypo-osmotic shock indicating that the linkage of the two polymers significantly contributes to the mechanical strength of the cell wall. Conversion of alkali-soluble glucan into alkali-insoluble glucan occurred both early and late during budding and also in the ts-mutant cdc24-1 in the absence of bud formation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Plný text