| Autor: |
Braman JC, Black MJ, Mangum JH |
| Jazyk: |
angličtina |
| Zdroj: |
Preparative biochemistry [Prep Biochem] 1981; Vol. 11 (1), pp. 23-32. |
| DOI: |
10.1080/00327488108068723 |
| Abstrakt: |
An improved radioactive assay has been developed for serine transhydroxymethylase. This assay involves the direct measurement of the [14C]HCHO which is generated when [3- 14C]-serine is employed as the substrate. The new assay eliminates the need for a solvent extraction of a [14C]HCHO-dimedon adduct which is the basis of the assay devised by Taylor and Weissbach. The enzyme has been purified employing Affi-Gel Blue. The purified enzyme retains full activity when bound to this affinity chromatography matrix and can be stored in this state at 4 degrees indefinitely. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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