Inactivation of rabies virus in reagents used for the fluorescent rabies antibody test.
| Autor: | White LA, Chappell WA |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Journal of clinical microbiology [J Clin Microbiol] 1982 Aug; Vol. 16 (2), pp. 253-6. |
| DOI: | 10.1128/jcm.16.2.253-256.1982 |
| Abstrakt: | Procedures for inactivating rabies virus in reagents used for the fluorescent rabies antibody test are described. Mouse brain adsorbing suspensions containing greater than or equal to 10(9) 50% lethal doses of virus per ml were rendered noninfectious by treatment with 0.1% beta-propiolactone or by heating at 56 degrees for greater than or equal to 30 min. Viable virus in tissue impression smears was inactivated by acetone fixation at 50 degrees C for greater than or equal to 30 min or by immersion in 0.1% beta-propiolactone at 37 degrees C for 2 h. Inactivated reagents gave specific and sensitive reactions in the fluorescent rabies antibody test. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|