[Role of Escherichia coli K-12 lambda phage receptors in transfection and plasmid transformation].

Autor: Likhacheva NA, Molchanova ES, Zakharenko VI, Il'iashenko BN
Jazyk: ruština
Zdroj: Genetika [Genetika] 1980; Vol. 16 (7), pp. 1176-81.
Abstrakt: The dependence of competence of Escherichia coli cells in transfection and plasmid transformation from phage lambda receptor protein was studied. Mal+ variants, sensitive to phage lambda (Mal+ lambda S) were obtained from strains with impaired lambda-phage receptor protein (Mal-lambda R). Maximum increase of transfection (4.7-fold) was observed in JC411Mal+ lambda S strain. The efficiency of plasmid transformation of pMB9 and RP4 DNAs was also higher in the strain with functioning lambda-receptor protein.
Databáze: MEDLINE