Enhancing reproducibility in single cell research with biocytometry: An inter-laboratory study.

Autor: Fikar P; Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America., Alvarez L; Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America., Berne L; Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America., Cienciala M; Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America., Kan C; Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America., Kasl H; Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America., Luo M; Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America., Novackova Z; Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America., Ordonez S; Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America., Sramkova Z; Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America., Holubova M; Department of Hematology and Oncology, University Hospital Pilsen, Pilsen, Czech Republic., Lysak D; Department of Hematology and Oncology, University Hospital Pilsen, Pilsen, Czech Republic., Avery L; Department of Biology, Saint Michael's College, Colchester, Vermont, United States of America., Caro AA; Chemistry Department, Hendrix College, Conway, Arkansas, United States of America., Crowder RN; Department of Biology, Stetson University, Deland, Florida, United States of America., Diaz-Martinez LA; Department of Biology, Gonzaga University, Spokane, Washington, United States of America., Donley DW; Department of Biology, Harding University, Searcy, Arkansas, United States of America., Giorno RR; School of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America., Reed IKG; Department of Biology, University of Saint Joseph, West Hartford, Connecticut, United States of America., Hensley LL; Department of Biological Sciences, Jacksonville State University, Jacksonville, Alabama, United States of America., Johnson KC; Department of Life Sciences, University of New Hampshire, Manchester, New Hampshire, United States of America., Kim AY; Department of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America., Kim P; Department of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America., LaGier AJ; Department of Biology, College of Social and Natural Sciences, Grand View University, Des Moines, Iowa, United States of America., Newman JJ; School of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America., Padilla-Crespo E; Department of Science and Technology, Inter American University of Puerto Rico-Aguadilla, Aguadilla, Puerto Rico, United States of America., Reyna NS; Department of Biology, Ouachita Baptist University, Arkadelphia, Arkansas, United States of America., Tsotakos N; Department of Biological Sciences, School of Science Engineering and Technology, Penn State Harrisburg, Middletown, Pennsylvania, United States of America., Al-Saadi NN; Department of Biological Sciences, Jacksonville State University, Jacksonville, Alabama, United States of America., Appleton T; Department of Biology, Harding University, Searcy, Arkansas, United States of America., Arosemena-Pickett A; Department of Science and Technology, Inter American University of Puerto Rico-Aguadilla, Aguadilla, Puerto Rico, United States of America., Bell BA; Department of Biology, Gonzaga University, Spokane, Washington, United States of America., Bing G; Department of Biology, Harding University, Searcy, Arkansas, United States of America., Bishop B; Department of Biology, Harding University, Searcy, Arkansas, United States of America., Forde C; Department of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America., Foster MJ; School of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America., Gray K; Department of Biology, Ouachita Baptist University, Arkadelphia, Arkansas, United States of America., Hasley BL; Department of Biological Sciences, Jacksonville State University, Jacksonville, Alabama, United States of America., Johnson K; Department of Biology, Ouachita Baptist University, Arkadelphia, Arkansas, United States of America., Jones DJ; Department of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America., LaShall AC; Department of Biological Sciences, Jacksonville State University, Jacksonville, Alabama, United States of America., McGuire K; Department of Biology, Ouachita Baptist University, Arkadelphia, Arkansas, United States of America., McNaughton N; Department of Biology, Harding University, Searcy, Arkansas, United States of America., Morgan AM; Department of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America., Norris L; School of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America., Ossman LA; School of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America., Rivera-Torres PA; Department of Science and Technology, Inter American University of Puerto Rico-Aguadilla, Aguadilla, Puerto Rico, United States of America., Robison ME; School of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America., Thibodaux K; School of Biological Sciences, Louisiana Tech University, Ruston, Louisiana, United States of America., Valmond L; Department of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America., Georgiev D; Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America.; Faculty of Applied Sciences, University of West Bohemia, Pilsen, Czech Republic.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Dec 09; Vol. 19 (12), pp. e0314992. Date of Electronic Publication: 2024 Dec 09 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0314992
Abstrakt: Biomedicine today is experiencing a shift towards decentralized data collection, which promises enhanced reproducibility and collaboration across diverse laboratory environments. This inter-laboratory study evaluates the performance of biocytometry, a method utilizing engineered bioparticles for enumerating cells based on their surface antigen patterns. In centralized and aggregated inter-lab studies, biocytometry demonstrated significant statistical power in discriminating numbers of target cells at varying concentrations as low as 1 cell per 100,000 background cells. User skill levels varied from expert to beginner capturing a range of proficiencies. Measurement was performed in a decentralized environment without any instrument cross-calibration or advanced user training outside of a basic instruction manual. The results affirm biocytometry to be a viable solution for immunophenotyping applications demanding sensitivity as well as scalability and reproducibility and paves the way for decentralized analysis of rare cells in heterogeneous samples.
Competing Interests: I have read the journal’s policy and the authors of this manuscript have the following competing interests: D.G., M.C., H.K and L.B. filed a patent related to biocytometry and are employees of Sampling Human. L.A., P.F., C.K., M.L., Z.N., S.O., Z.S. are employees at Sampling Human and are working to commercialize the biocytometry technology. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
(Copyright: © 2024 Fikar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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