Pulsed radiofrequency alleviates neuropathic pain by upregulating MG53 to inhibit microglial activation.

Autor: Huang Y; Pain Department, Affiliated Hospital of Guizhou Medical University, No. 28, Guiyi Street, Liu Guang Men, Guiyang, 550004, Guizhou, China., Huang Y; Clinical Medicine School, Guizhou Medical University, Guiyang, 550004, Guizhou, China., Lv X; Clinical Medicine School, Guizhou Medical University, Guiyang, 550004, Guizhou, China., Yu Z; Pain Department, Affiliated Hospital of Guizhou Medical University, No. 28, Guiyi Street, Liu Guang Men, Guiyang, 550004, Guizhou, China., Qin Y; Pain Department, Affiliated Hospital of Guizhou Medical University, No. 28, Guiyi Street, Liu Guang Men, Guiyang, 550004, Guizhou, China., Yang X; Clinical Medicine School, Guizhou Medical University, Guiyang, 550004, Guizhou, China., An S; Clinical Medicine School, Guizhou Medical University, Guiyang, 550004, Guizhou, China., Wo C; Pain Department, Affiliated Hospital of Guizhou Medical University, No. 28, Guiyi Street, Liu Guang Men, Guiyang, 550004, Guizhou, China. wochunxin@163.com., Wang L; Pain Department, Affiliated Hospital of Guizhou Medical University, No. 28, Guiyi Street, Liu Guang Men, Guiyang, 550004, Guizhou, China. wanglin0949@yeah.com.
Jazyk: angličtina
Zdroj: European journal of medical research [Eur J Med Res] 2024 Dec 05; Vol. 29 (1), pp. 578. Date of Electronic Publication: 2024 Dec 05.
DOI: 10.1186/s40001-024-02134-6
Abstrakt: Background: Patients with neuropathic pain (NP) have significantly lower quality of life. Because the pathophysiology of NP is not fully understood, there is a lack of effective treatment for it in clinic. This study set out to investigate the precise mechanism by which pulsed radiofrequency (PRF) alleviated NP.
Method: The rat models of chronic constriction injury of the sciatic nerve (CCI) were established to simulate the occurrence of NP, following with measuring MWT and TWL to evaluate the pain of the rats. HE staining was utilized to observe the rat spinal cord tissue pathology. The expression of MG53, ATF4 and CHOP was evaluated by qRT-PCR and WB, while the expression of inflammatory factors was measured by ELISA. In addition, immunofluorescence assay was used to detect the expression of MG53 and Iba-1.
Result: PRF treatment alleviated NP in CCI rats, as well as upregulating the expression of MG53 and inhibiting microglial activation. After MG53 knockdown, the remission of NP by PRF was significantly weakened, but microglial activation and endoplasmic reticulum stress (ERS) exhibited enhancement. Therefore, PRF inhibited microglial activation by upregulating MG53. After injection of ERS inducer in CCI rats, the inhibition effect of overexpressed MG53 on microglial activation and its alleviation effect on NP were reversed. Consequently, MG53 played a role in suppressing microglial activation by mediating the inhibition of ERS.
Conclusion: PRF attenuated microglial activation by upregulating MG53 to inhibit ERS, resulting in the alleviation of NP in CCI rats.
Competing Interests: Declarations. Ethics approval and consent to participate: All animal experimental procedures were performed in accordance with the Institutional Animal Care and Use Committee of Affiliated Hospital of Guizhou Medical University, which was approved by the Institutional Animal Care and Use Committee of Affiliated Hospital of Guizhou Medical University. All methods were performed under the relevant guidelines and ARRIVE guidelines. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: