Combined transcriptome and metabolome analysis revealed the antimicrobial mechanism of Griseorhodin C against Methicillin-resistant Staphylococcus aureus.

Autor: Liao J; School of basic medical sciences, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China., Tan J; School of basic medical sciences, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China., Li X; School of basic medical sciences, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China., Huang D; School of basic medical sciences, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China., Wang L; School of basic medical sciences, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China., Zhu L; School of basic medical sciences, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China., Chen Z; School of basic medical sciences, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China., Gan L; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China., Liu W; School of basic medical sciences, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China. 408011126@gdpu.edu.cn.; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China. 408011126@gdpu.edu.cn., Jin X; School of basic medical sciences, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China. jinxf2001@163.com.; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China. jinxf2001@163.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Dec 04; Vol. 14 (1), pp. 30242. Date of Electronic Publication: 2024 Dec 04.
DOI: 10.1038/s41598-024-76212-4
Abstrakt: The global rise of multidrug-resistant pathogens, particularly Methicillin-resistant Staphylococcus aureus (MRSA), has become a critical public health concern, necessitating the urgent discovery of new antimicrobial agents. Griseorhodin C, a hydroxyquinone compound isolated from Streptomyces, has demonstrated significant inhibitory effects against MRSA. In this study, we employed a comprehensive approach combining transcriptome and metabolome analyses to investigate the underlying antimicrobial mechanism of Griseorhodin C. Our findings reveal that Griseorhodin C interferes with multiple bacterial metabolic pathways, including those essential for the biosynthesis and metabolism of amino acids, purine metabolism and energy metabolism, ultimately leading to bacterial growth inhibition and cell death. Notably, Griseorhodin C showed superior inhibitory effects compared to the clinical standard, vancomycin, both in vivo and vitro. These results highlight the potential of Griseorhodin C as a promising candidate for the development of new therapeutic strategies aimed at combating MRSA infections. The study underscores the importance of exploring natural products as sources of novel antibiotics in the ongoing fight against antimicrobial resistance.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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