WHIRLY1 regulates aliphatic glucosinolate biosynthesis in early seedling development of Arabidopsis.

Autor: Nguyen LT; Institute of Biology, Martin-Luther-University Halle-Wittenberg, 06120, Halle (Saale), Germany., Moutesidi P; Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry (IPB), 06120, Halle (Saale), Germany., Ziegler J; Program Center for Plant Metabolomics and Computational Biochemistry, Leibniz Institute of Plant Biochemistry (IPB), 06120, Halle (Saale), Germany., Glasneck A; Institute of Botany, Christian-Albrechts-University (CAU), 24098, Kiel, Germany., Khosravi S; Department of Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, 06466, Seeland, Germany., Abel S; Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry (IPB), 06120, Halle (Saale), Germany., Hensel G; Centre for Plant Genome Engineering, Institute of Plant Biochemistry, Heinrich-Heine-University Duesseldorf, 40225, Duesseldorf, Germany., Krupinska K; Institute of Botany, Christian-Albrechts-University (CAU), 24098, Kiel, Germany., Humbeck K; Institute of Biology, Martin-Luther-University Halle-Wittenberg, 06120, Halle (Saale), Germany.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2024 Dec 03. Date of Electronic Publication: 2024 Dec 03.
DOI: 10.1111/tpj.17181
Abstrakt: WHIRLY1 belongs to a family of plant-specific transcription factors capable of binding DNA or RNA in all three plant cell compartments that contain genetic materials. In Arabidopsis thaliana, WHIRLY1 has been studied at the later stages of plant development, including flowering and leaf senescence, as well as in biotic and abiotic stress responses. In this study, WHIRLY1 knockout mutants of A. thaliana were prepared by CRISPR/Cas9-mediated genome editing to investigate the role of WHIRLY1 during early seedling development. The loss-of-function of WHIRLY1 in 5-day-old seedlings did not cause differences in the phenotype and the photosynthetic performance of the emerging cotyledons compared with the wild type. Nevertheless, comparative RNA sequencing analysis revealed that the knockout of WHIRLY1 affected the expression of a small but specific set of genes during this critical phase of development. About 110 genes were found to be significantly deregulated in the knockout mutant, wherein several genes involved in the early steps of aliphatic glucosinolate (GSL) biosynthesis were suppressed compared with wild-type plants. The downregulation of these genes in WHIRLY1 knockout lines led to decreased GSL contents in seedlings and in seeds. Since GSL catabolism mediated by myrosinases was not altered during seed-to-seedling transition, the results suggest that AtWHIRLY1 plays a major role in modulation of aliphatic GSL biosynthesis during early seedling development. In addition, phylogenetic analysis revealed a coincidence between the evolution of methionine-derived aliphatic GSLs and the addition of a new WHIRLY in core families of the plant order Brassicales.
(© 2024 The Author(s). The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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