Zingiber officinale Uncovered: Integrating Experimental and Computational Approaches to Antibacterial and Phytochemical Profiling.

Autor: Sulieman AME; Department of Biology, College of Science, University of Ha'il, Ha'il 81451, Saudi Arabia., Ibrahim SM; Department of Microbiology, Faculty of Sciences, University of Gezira, Wad-Medani 21111, Sudan., Alshammari M; Department of Biology, College of Science, University of Ha'il, Ha'il 81451, Saudi Arabia., Abdulaziz F; Department of Chemistry, College of Science, University of Ha'il, Ha'il 81451, Saudi Arabia., Idriss H; Department of Physics, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11432, Saudi Arabia.; Deanship of Scientific Research, Imam Mohammad Ibn Saud Islamic University (IMSIU), P.O. Box 5701, Riyadh 11432, Saudi Arabia., Alanazi NAH; Department of Biology, College of Science, University of Ha'il, Ha'il 81451, Saudi Arabia., Abdallah EM; Department of Biology, College of Science, Qassim University, Buraydah 51452, Saudi Arabia., Siddiqui AJ; Department of Biology, College of Science, University of Ha'il, Ha'il 81451, Saudi Arabia., Shommo SAM; Department of Sport Science and Physical Activity, College of Education, University of Ha'il, Ha'il 81451, Saudi Arabia., Jamal A; Department of Biology, College of Science, University of Ha'il, Ha'il 81451, Saudi Arabia., Badraoui R; Department of Biology, College of Science, University of Ha'il, Ha'il 81451, Saudi Arabia.; Section of Histology-Cytology, Medicine Faculty of Tunis, University of Tunis El Manar, La Rabta, Tunis 1007, Tunisia.
Jazyk: angličtina
Zdroj: Pharmaceuticals (Basel, Switzerland) [Pharmaceuticals (Basel)] 2024 Nov 19; Vol. 17 (11). Date of Electronic Publication: 2024 Nov 19.
DOI: 10.3390/ph17111551
Abstrakt: Background: Zingiber officinale rhizome is widely cultivated in the central region of Sudan (Gezira) and data on the biological properties of this variety grown in Sudan's climate are scarce. This study aims to comprehensively analyze the antibacterial, antioxidant, phytochemical, and GC-MS properties of Zingiber officinale (ginger rhizome) to explore its potential applications.
Methods and Results: The in vitro antibacterial assessment of the aqueous extract of Sudanese ginger revealed moderate activity against Staphylococcus aureus , Salmonella typhi , Pseudomonas aeruginosa , Escherichia coli , and Klebsiella pneumonia , as determined by the disc diffusion method. The inhibition zones ranged from 12.87 ± 0.11 mm to 14.5 ± 0.12 mm at 30 µg/disc. The minimum inhibitory concentration ranged from 6.25 to 25 µg/mL, while the MBC ranged from 25 to 50 µg/mL. The MBC/MIC exhibited a bactericidal effect against all tested bacteria. Phytochemical screening revealed the presence of various chemical constituents, such as saponins, flavonoids, glycosides, alkaloids, steroids, terpenoids, and the absence of tannins in Sudanese ginger rhizome. Furthermore, GC-MS analysis of ginger rhizome identified 22 chemical compounds with retention times ranging from 7.564 to 17.023 min. The identification of 22 chemical compounds through GC-MS analysis further underscores the prospect of harnessing ginger rhizome for the development of novel medications. Computational analyses showed that ginger compounds bind the Protein Data Bank (PDB) codes 1JIJ and 2QZW with high binding affinities, reaching -9.5 kcal/mol. Ginger compounds also established promising molecular interactions with some key residues, satisfactorily explaining the in vitro results and supporting the pharmacokinetic and experimental findings.
Conclusions: This study lays the groundwork for future research and pharmaceutical exploration aimed at harnessing the beneficial properties of ginger rhizome for medicinal and therapeutic purposes, particularly its antimicrobial potential.
Databáze: MEDLINE
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