Gene Expression Modulation of Markers Involved in Bone Formation and Resorption by Bisphenol A, Bisphenol F, Bisphenol S, and Bisphenol AF.
| Autor: | García-Recio E; Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, Spain.; Institute of Biosanitary Research, ibs.Granada, Avda. de Madrid 15, Pabellón de Consultas Externas, 2ª Planta, 18012 Granada, Spain., González-Acedo A; Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, Spain.; Institute of Biosanitary Research, ibs.Granada, Avda. de Madrid 15, Pabellón de Consultas Externas, 2ª Planta, 18012 Granada, Spain., Manzano-Moreno FJ; Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, Spain.; Biomedical Group (BIO277), Department of Stomatology, School of Dentistry, University of Granada, 18016 Granada, Spain., De Luna-Bertos E; Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, Spain.; Institute of Biosanitary Research, ibs.Granada, Avda. de Madrid 15, Pabellón de Consultas Externas, 2ª Planta, 18012 Granada, Spain., Ruiz C; Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, Spain.; Institute of Biosanitary Research, ibs.Granada, Avda. de Madrid 15, Pabellón de Consultas Externas, 2ª Planta, 18012 Granada, Spain.; Institute of Neuroscience, University of Granada, 18016 Granada, Spain. |
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| Jazyk: | angličtina |
| Zdroj: | Genes [Genes (Basel)] 2024 Nov 11; Vol. 15 (11). Date of Electronic Publication: 2024 Nov 11. |
| DOI: | 10.3390/genes15111453 |
| Abstrakt: | Background: Bisphenol A (BPA) and its analogs (BPF, BPS, and BPAF) are recognized for inducing detrimental effects on various tissues, including bone. Objectives: The aim of this study is to investigate their impact on information and repair processes, specifically focusing on vascular endothelial growth factor (VEGF), transforming growth factor β1 (TGF-β1), and the receptors for transforming growth factor β (TGFR1, TGFR2, and TGFR3). Methods: Human osteoblasts isolated through primary culture from bone samples of healthy volunteers were subjected to cultivation in the presence of various dosage levels (10 -5 , 10 -6 , or 10 -7 M) of BPA, BPF, BPS, or BPAF for 24 h. Gene expressions of RANKL, OPG, TGF-β1, TGFR1, TGFR2, TGFR3, and VEGF were analyzed by real-time polymerase chain reaction (RT-PCR). All experiments included untreated cells as controls. Results: Expressions of RANKL and OPG were dose-dependently downregulated by the presence of all tested bisphenols (BPs) except for BPAF, whose presence upregulated OPG expression at all three doses. TGF-β1 expression was downregulated by all BP treatments, and TGF-β1 receptor expression was also downregulated as a function of the BP and dose. VEGF expression was downregulated in the presence of BPF and BPAF at all three doses and in the presence of BPA at the two higher doses (10 -5 , and 10 -6 M), but it was not changed by the presence of BPS at any dose. Conclusions: The inhibition of both RANKL and OPG by the BPs, with a higher %inhibition of RANKL than of OPG, appears to rule out BP-induced activation of osteoclastogenesis via RANKL/RANK/OPG. Nevertheless, the effect of the BPs on the expression by osteoblasts of TGF-β1, TGF-β receptors, and VEGF indicates that these compounds can be responsible for major molecular changes in this cell population, contributing to their adverse effects on bone tissue. |
| Databáze: | MEDLINE |
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