Impact of Wastewater Use for Irrigation and Contamination of Lettuce by Enteric Viruses: Case of Ouagadougou Market Gardening Sites, Burkina Faso.

Autor: Rouamba SS; National Public Health Laboratory, 09 BP 24, 09, Ouagadougou, Burkina Faso. rousib@yahoo.fr.; Applied Biochemistry and Immunology Laboratory (LaBIA), Sciences and Technologies Doctoral School, KI-ZERBO University, 03 BP 7021 03, 03, Ouagadougou, Burkina Faso. rousib@yahoo.fr., Tapsoba F; Applied Biochemistry and Immunology Laboratory (LaBIA), Sciences and Technologies Doctoral School, KI-ZERBO University, 03 BP 7021 03, 03, Ouagadougou, Burkina Faso., Kaboré B; Applied Biochemistry and Immunology Laboratory (LaBIA), Sciences and Technologies Doctoral School, KI-ZERBO University, 03 BP 7021 03, 03, Ouagadougou, Burkina Faso. Kaboreboukare27@gmail.com., Soubeiga AP; National Public Health Laboratory, 09 BP 24, 09, Ouagadougou, Burkina Faso.; Applied Biochemistry and Immunology Laboratory (LaBIA), Sciences and Technologies Doctoral School, KI-ZERBO University, 03 BP 7021 03, 03, Ouagadougou, Burkina Faso., Bandé M; National Public Health Laboratory, 09 BP 24, 09, Ouagadougou, Burkina Faso., Kabré E; National Public Health Laboratory, 09 BP 24, 09, Ouagadougou, Burkina Faso.; Training and Research Unit/Health Sciences (UFR/SDS), KI-ZERBO University, 03 BP: 7021, 03, Ouagadougou, Burkina Faso., Savadogo A; Applied Biochemistry and Immunology Laboratory (LaBIA), Sciences and Technologies Doctoral School, KI-ZERBO University, 03 BP 7021 03, 03, Ouagadougou, Burkina Faso.
Jazyk: angličtina
Zdroj: Food and environmental virology [Food Environ Virol] 2024 Nov 24; Vol. 17 (1), pp. 2. Date of Electronic Publication: 2024 Nov 24.
DOI: 10.1007/s12560-024-09621-3
Abstrakt: Raw vegetables irrigated with polluted water that may contain enteric viruses can be associated with foodborne viral disease outbreaks. The objective of this study is to investigate the possible transmission of enteric viruses from irrigation water to lettuce. Therefore, we performed a commercial multiplex real-time PCR assay to monitor the occurrence of enteric viruses in irrigation water samples and in raw vegetables that were cultivated at market gardening sites in Ouagadougou, Burkina Faso. Samples were collected from six market gardening sites located in Ouagadougou. RT-PCR was performed to detect norovirus GI, norovirus GII, rotavirus, enteric adenoviruses F (Serotype 40/41), astrovirus and sapovirus (Genogroups G1, 2, 4, 5). From the 10 irrigation water samples and the 80 lettuce samples, three (30%) and twenty-two (27.5%) were positive for enteric viruses, respectively. Norovirus GII, astrovirus and enteric adenoviruses F (Serotype 40/41) were the most frequently detected viruses in lettuce and irrigation water samples. Our results indicate that raw vegetables may be contaminated with a broad range of enteric viruses, which may originate from virus-contaminated irrigation water, and these vegetables may act as a potential vector of food-borne viral transmission.
Competing Interests: Declarations. Competing Interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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