Application of Liquid Chromatography-Ion Mobility Spectrometry-Mass Spectrometry-Based Metabolomics to Investigate the Basal Chemical Profile of Olive Cultivars Differing in Verticillium dahliae Resistance.

Autor: Serrano-García I; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Ave. Fuentenueva s/n, Granada 18071, Spain., Martakos IC; Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, Athens 15771, Greece.; Food Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, Athens 15771, Greece., Olmo-García L; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Ave. Fuentenueva s/n, Granada 18071, Spain., León L; IFAPA Centro Alameda del Obispo, Av. Menéndez Pidal s/n, Córdoba 14004, Spain., de la Rosa R; IFAPA Centro Alameda del Obispo, Av. Menéndez Pidal s/n, Córdoba 14004, Spain.; Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Av. Menéndez Pidal s/n, Córdoba 14004, Spain., Gómez-Caravaca AM; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Ave. Fuentenueva s/n, Granada 18071, Spain., Belaj A; IFAPA Centro Alameda del Obispo, Av. Menéndez Pidal s/n, Córdoba 14004, Spain., Serrano A; The University Institute of Research into Olives and Olive Oils (INUO), University of Jaén, Campus Las Lagunillas s/n, Jaén 23071, Spain., Dasenaki ME; Food Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, Athens 15771, Greece., Thomaidis NS; Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, Athens 15771, Greece., Carrasco-Pancorbo A; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Ave. Fuentenueva s/n, Granada 18071, Spain.
Jazyk: angličtina
Zdroj: Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Nov 22. Date of Electronic Publication: 2024 Nov 22.
DOI: 10.1021/acs.jafc.4c07155
Abstrakt: The limited effectiveness of current strategies to control Verticillium wilt of olive (VWO) prompts the need for innovative approaches. This study explores the basal metabolome of 43 olive cultivars with varying resistance levels to Verticillium dahliae , offering alternative insights for olive crossbreeding programmes. The use of an innovative UHPLC-ESI-TimsTOF MS/MS platform enabled the annotation of more than 70 compounds across different olive organs (root, stem, and leaf) and the creation of a preliminary compilation of TIMS CCS N2 experimental data for more reliable metabolite annotation. Moreover, it allowed the documentation of numerous isomeric species in the studied olive organs by resolving hidden compounds. Multivariate statistical analyses revealed significant metabolome variability between highly resistant and susceptible cultivars, which was further investigated through supervised PLS-DA. Key markers indicative of VWO susceptibility were annotated and characteristic compositional patterns were established. Stem tissue exhibited the highest discriminative capability, while root and leaf tissues also showed significant predictive potential.
Databáze: MEDLINE