Genomic and taxonomic characterization of the Comamonas sp. nov., a bacterium isolated from Brazilian Cerrado soil.

Autor: Frederico TD; Laboratory of Enzymology, Department of Cellular Biology, University of Brasília (UNB), Brasília, Brazil., Cunha-Ferreira IC; Laboratory of Enzymology, Department of Cellular Biology, University of Brasília (UNB), Brasília, Brazil., Vizzotto CS; Laboratory of Environmental Sanitation, Department of Civil and Environmental Engineering, University of Brasília (UNB), Brasília, Brazil., de Sousa JF; Laboratory of Enzymology, Department of Cellular Biology, University of Brasília (UNB), Brasília, Brazil., Portugal MM; Laboratory of Enzymology, Department of Cellular Biology, University of Brasília (UNB), Brasília, Brazil., Tótola MR; Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente, Universidade Federal de Viçosa (UFV), Viçosa, Brazil., Krüger RH; Laboratory of Enzymology, Department of Cellular Biology, University of Brasília (UNB), Brasília, Brazil. kruger@unb.br., Peixoto J; Laboratory of Enzymology, Department of Cellular Biology, University of Brasília (UNB), Brasília, Brazil. julianna.peixoto@mail.huji.ac.il.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Nov 22. Date of Electronic Publication: 2024 Nov 22.
DOI: 10.1007/s42770-024-01566-w
Abstrakt: A novel strain identified as Comamonas sp. was isolated from the soil of the Brazilian savanna-like Cerrado biome, a global hotspot for biodiversity. Phylogenetic analysis based on 16 S rRNA gene sequences showed that this strain is classified as Betaproteobacteria from the family Comamonadaceae. The digital DNA-DNA hybridization (dDDH) and Average Nucleotide Identity (ANI) results, of respectively 48.6% and < 93%, indicated that Comamonas sp. consists in a new species with Comamonas testosteroni as its closest strain. Comamonas sp. is a Gram-negative, rod-shaped, and non-spore-forming bacterium. Its colonies typically exhibit a round, convex, and irregular shape with a clear color and spotted edges. It is characterized as non-fermenting, aerobic, and motile, presenting both oxidase and catalase activities. The optimal growth parameters for this bacterial strain are 30 °C, a pH range of 5-8, and 0% NaCl. In addition, its fatty acid profile included palmitic acid (C 16:0 ) at 26.94%, 13-Methyltetradecanoic Acid (iso-C 15:0 ) at 10.94%, myristic acid (C 14:0 ) at 8.94%, and a summed feature comprising 16:1 ω7c, 16:1 ω6c, or 16:1 at 15.8%. Genomic analysis of Comamonas sp. revealed a GC content of 62.1% across its 5.6 Mb genome. Phylogenomic and pangenome analyses, along with in silico phenotypic characterization indicate that this strain represents a novel species within the Comamonas genus, which we propose to name Comamonas brasiliensis nov.
Competing Interests: Declarations. Conflict of interest: The authors declare that there are no conflicts of interest regarding the publication of this paper.
(© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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