Efficacy of Lactobacillus acidophilus and yeast cell wall-derived supplements on immunity responses, growth performance, and disease resistance in Cyprinus carpio juveniles.

Autor: Ghafarifarsani H; Department of Animal Science, Chaharmahal and Bakhtiari Agricultural and Natural Resources Research and Education Center, AREEO, Shahrekord, Iran. hamed_ghafari@alumni.ut.ac.ir., Ahani S; School of Veterinary Medicine, Islamic Azad University Karaj Branch, Karaj, Iran., Aftabgard M; Young Researchers and Elite Club, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, Iran., Ahani S; Department of Fisheries, College of Agricultural and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran., Yousefi M; Department of Veterinary Medicine, RUDN University, 6 Miklukho-Maklaya St., Moscow, 117198, Russia. myousefi81@gmail.com.
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2024 Nov 21; Vol. 49 (1), pp. 23. Date of Electronic Publication: 2024 Nov 21.
DOI: 10.1007/s11259-024-10567-x
Abstrakt: This study investigated the effects of probiotic Lactobacillus acidophilus (PTCC 1643) (LA) and a commercial yeast cell wall prebiotic (Immunogen ® ) (IM) on immunoantioxidant, growth, and digestive status, and resistance to Aeromonas hydrophila in Cyprinus carpio juveniles. The fish were fed in five treatments including control (T1), LA1.5 (T2): 1.5 (× 10^8 CFU/g of LA)/kg diet, LA3 (T3): 3 (× 10^8 CFU/g of LA)/kg diet, IM1 (T4): 1 g of IM/kg diet, and IM2 (T5): 2 g of IM/ kg diet. After 60 days, the function of growth and intestinal protease and amylase showed a significant increase in IM treatments. Serum levels of total protein and superoxide dismutase in LA3 treatment, as well as albumin, catalase, and glutathione peroxidase in LA3 and IM2 treatments were significantly increased. Serum level of malondialdehyde in LA and IM treatments, as well as alkaline phosphatase (ALP) in LA treatments, alanine aminotransferase in LA and IM2 treatments, and lactate dehydrogenase in LA3 treatment was significantly decreased. Serum levels of all the immune parameters in LA3 treatment, as well as alternative complement pathway hemolytic and nitroblue tetrazolium in IM2 treatment were significantly improved. Mucosal level of protease in LA3 and IM2 treatments, total immunoglobulin, and lysozyme in LA and IM2 treatments, as well as ALP and peroxidase in LA3 treatment was significantly increased. Cumulative mortality rate in LA and IM treatments was significantly decreased compared to control after the 14-day challenge with A. hydrophila. It is recommended to include IM2 in the diet of C. carpio juveniles with regard to its beneficial effects on growth and immunity status.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Institutional review board statement: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All experiments were performed following the protocol approved by the committee of ethics of the Baharavaran Nastaran Agricultural Applied Scientific Training Center, Applied Scientific University, Qom, Iran (1087; 2023). Informed consent: Not applicable.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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