Syngas biological transformation into hydroxyectoine.

Autor: Marcos-Rodrigo E; Institute of Sustainable Processes, Doctor Mergelina s/n, Valladolid 47011, Spain; Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Doctor Mergelina s/n, Valladolid 47011, Spain., Lebrero R; Institute of Sustainable Processes, Doctor Mergelina s/n, Valladolid 47011, Spain; Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Doctor Mergelina s/n, Valladolid 47011, Spain., Muñoz R; Institute of Sustainable Processes, Doctor Mergelina s/n, Valladolid 47011, Spain; Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Doctor Mergelina s/n, Valladolid 47011, Spain., Sousa DZ; Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, the Netherlands., Cantera S; Institute of Sustainable Processes, Doctor Mergelina s/n, Valladolid 47011, Spain; Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Doctor Mergelina s/n, Valladolid 47011, Spain. Electronic address: sara.cantera@uva.es.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2024 Nov 19; Vol. 417, pp. 131842. Date of Electronic Publication: 2024 Nov 19.
DOI: 10.1016/j.biortech.2024.131842
Abstrakt: Syngas from the gasification of organic wastes represents a promising feedstock for fostering a sustainable bioeconomy. However, its potential is currently constrained by the low-value products generated. Osmolytes, such as hydroxyectoine, are high-value compounds, however, their biological production as isolated osmolytes is not yet cost-effective. This study utilized shotgun genomics and laboratory validation to find a carboxydotrophic, halotolerant bacterium, Hydrogenibacillus schlegelii, that could produce hydroxyectoine using H 2 , CO and CO 2 as the sole source of energy and carbon. Subsequently, NaCl concentration, temperature and syngas composition were optimized in semi-continuous bioreactors. Optimal conversion of CO into hydroxyectoine occurred at a gas composition of 70 %:10 % CO:H 2 (v/v) (44.8 ± 10.1 mg hydroxyectoine ·g biomass -1 ). NaCl concentrations of 5 % significantly enhanced hydroxyectoine content (46.7 ± 9.5 mg hydroxyectoine ·g biomass -1 ), but negatively affected gas consumption. This study opens new perspectives for the valorisation of syngas into hydroxyectoine, and for new cell platforms for pharmaceutical production based on syngas.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: