Changes in fatty acids, vitamins, cholesterol and amino acid profiles of ram semen by freeze-thawing process.

Autor: Güngör İH; Fırat University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Elazığ, Turkey,. Electronic address: ihgungor@firat.edu.tr., Koca RH; Bingöl University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Bingöl, Turkey., Cinkara SD; Fırat University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Elazığ, Turkey., Acısu TC; Fırat University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Elazığ, Turkey., Erişir FE; Fırat University, Faculty of Science, Department of Biology, Elazığ, Turkey., Arkalı G; Fırat University, Faculty of Veterinary Medicine, Department of Physiology, Elazığ, Turkey., Kaya ŞÖ; Fırat University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Elazığ, Turkey., Sönmez M; Fırat University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Elazığ, Turkey., Gür S; Fırat University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Elazığ, Turkey., Yılmaz Ö; Fırat University, Faculty of Science, Department of Biology, Elazığ, Turkey., Yüce A; Fırat University, Faculty of Veterinary Medicine, Department of Physiology, Elazığ, Turkey., Türk G; Fırat University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Elazığ, Turkey.
Jazyk: angličtina
Zdroj: Reproductive biology [Reprod Biol] 2024 Nov 19; Vol. 25 (1), pp. 100953. Date of Electronic Publication: 2024 Nov 19.
DOI: 10.1016/j.repbio.2024.100953
Abstrakt: The objective of this study was to examine the impact of freeze-thawing on the levels of oxidative stress, fatty acids, vitamins A, D, E, and K, cholesterol, and amino acids, as well as on spermatological parameters, in ram semen. Semen was collected and pooled from each of the seven rams twice a week for three weeks. The mixed semen was diluted with tris + egg yolk diluent at 38 °C (Group 38 °C) and the temperature was reduced to 5 °C (Group 5 °C). Following the glycerolization-equilibration process (Group G-E), the samples were automatically frozen in liquid nitrogen vapor at -140 °C. The semen samples were thawed 24 h after freezing (Group Frozen-Thawed, F-T). A comparison of Group 38 °C with Group F-T revealed significant differences in several parameters. Motility rates, kinematic values, percentage of membrane integrity (HOS), some PUFA levels, ∑SFA and amino acid levels were significantly lower in Group F-T. Conversely, the ratio of dead and static spermatozoa, lipid peroxidation level, some PUFA levels, ∑MUFA, vitamins A, E and cholesterol levels were significantly higher in Group F-T. The majority of these alterations were also evident in semen samples subjected to G-E treatment. In conclusion, exposure of ram semen to G-E and F-T treatments results in modifications to semen, fatty acid, vitamin, and amino acid profiles, accompanied by elevated levels of lipid peroxidation. Moreover, this study demonstrated, for the first time, that oxidative stress was induced, some amino acid levels were altered, vitamin A and E levels were increased, vitamin D and K levels were not affected, and β-sitosterol levels were decreased after freeze-thawing in ram semen.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
(Copyright © 2024 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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