An aptamer and hydroxyapatite-silver-zinc oxide-based novel electrochemical sensor for ultrasensitive H. pylori detection.

Autor: Rafique S; Department of Physics, Air University, PAF Complex, E-9, Islamabad, 44000, Pakistan. saima.rafique@mail.au.edu.pk., Akram R; Department of Physics, Air University, PAF Complex, E-9, Islamabad, 44000, Pakistan., Nasir R; Department of Physics, Air University, PAF Complex, E-9, Islamabad, 44000, Pakistan., Naz N; Department of Physics, Air University, PAF Complex, E-9, Islamabad, 44000, Pakistan., Shafique A; Department of Physics, Air University, PAF Complex, E-9, Islamabad, 44000, Pakistan., Bashir S; Department of Physics and Applied Mathematics, Pakistan Institute of Engineering and Applied Sciences, Islamabad, 45650, Pakistan., Haq Z; Department of Biosciences, University of Wah, Wah Cantt, Pakistan.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Nov 20; Vol. 191 (12), pp. 751. Date of Electronic Publication: 2024 Nov 20.
DOI: 10.1007/s00604-024-06834-y
Abstrakt: Electrochemical biosensors offer a cost-effective way to identify Helicobacter pylori (H. pylori) (Hsp 60), responsible for stomach infections. The conductivity and surface area of the electrode were enhanced using Hap-Ag-ZnO composites on glassy carbon electrode (GCE) along with polythiophene (PP), as a conductive polymer to improve proficiency. The Hap-Ag-ZnO-PP composites have multiple functional group sites that facilitate aptamer immobilization on the GCE. This increases the sensitivity, stability, and dynamic range of the developed aptasensor. Electrochemical impedance spectroscopy, cyclic voltammetry, and square wave voltammetry were employed to examine the performance of the aptasensor. A linear behavior was observed for the modified electrode against the concentration of Hsp 60, showing a detection limit of 0.429 nM and a wide dynamic range of 0.05-300 nM. Moreover, the Hap-Ag-ZnO-PP-based aptasensor exhibits exceptional reproducibility, repeatability, fast response time (20 min), good selectivity, and excellent stability (electrochemical and storage). Therefore, the prepared sensitive aptasensor provides a promising platform for clinical diagnostics and H. pylori detection in real-world applications.
Competing Interests: Declarations. Ethical approval: The NIH (National Institute of Health) Islamabad, Pakistan, provided standard laboratory procedures and guidelines, followed when collecting blood serum samples from study participants and healthy volunteers. The ethical standards described in the Declaration of Helsinki, which are accepted nationally and internationally, serve as the foundation for these regulations. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: