| Autor: |
Dang HH; Faculty of Pharmacy, PHENIKAA University, Hanoi, Vietnam., Doan MD; Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, DakLak, Vietnam., Dang TN; University of Medicine and Pharmacy, Vietnam National University, Hanoi (VNU), Cau Giay, Hanoi, Vietnam., Nguyen THA; University of Medicine and Pharmacy, Vietnam National University, Hanoi (VNU), Cau Giay, Hanoi, Vietnam., Phuong TT; Division of Biotechnology, Vietnam-Korea Institute of Science and Technology, Hoa Lac High Tech Park, Hanoi, Vietnam., Vu Van T; Faculty of Pharmacy, PHENIKAA University, Hanoi, Vietnam., Nguyen Ngoc H; Faculty of Pharmacy, PHENIKAA University, Hanoi, Vietnam., Nguyen HT; Faculty of Pharmacy, PHENIKAA University, Hanoi, Vietnam. |
| Abstrakt: |
Majonoside R2 (MR2), the principal saponin of Vietnamese ginseng ( Panax vietnamensis Ha & Grushv.), has the unique structure of ocotillol-type dammarane and showed remarkable biological activities. This paper deals with the new findings in the chemical analysis MR2 by the tandem LC-MS/MS and, especially, its inhibitory activities on α -glucosidase for diabetic management. The developed LC-MS/MS method revealed advantages of high selectivity with specific mass transition from precursor [M + H] + ion ( m/z 784.4) into product ion ( m/z 475.1), high sensitivity (calibration range: 5-250 ppb; LOD: 1.5 ppb; LOQ: 5.0 ppb), and high accuracy to support further pharmaceutical analysis of MR2. MR2 and its aglycone ocotillol relatively showed certain inhibitory effects on α -glucosidase in vitro with the IC 50 values of 353.05 and 219.64 µg/mL and supported by molecular docking analysis, in which MR2 and ocotillol could play as allosteric inhibitors with high binding affinity (-7.8 and -8.1 kcal/mol) evidenced by bonding interactions. |
