Dose sparing enabled by immunization with influenza vaccine using orally dissolving film.

Autor: Yoon KW; Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea., Chu KB; Department of Parasitology, Inje University College of Medicine, Busan 47392, Republic of Korea; Department of Infectious Disease and Malaria, Paik Institute of Clinical Research, Inje University, Busan 47392, Republic of Korea., Eom GD; Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea., Mao J; Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea., Heo SI; Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea., Quan FS; Department of Medical Zoology, School of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea; Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, Core Research Institute (CRI), Kyung Hee University, Seoul 02447, Republic of Korea. Electronic address: fsquan@khu.ac.kr.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2024 Dec 25; Vol. 667 (Pt B), pp. 124945. Date of Electronic Publication: 2024 Nov 15.
DOI: 10.1016/j.ijpharm.2024.124945
Abstrakt: Influenza vaccine delivered by orally dissolving film vaccine (ODFV) is a promising approach. In this study, we generated three ODFVs each comprising pulluan and trehalose with different doses of inactivated A/Puerto Rico/8/34, H1N1 virus (ODFV I, II, III) to evaluate their dose-sparing effect in mice. The ODFVs were placed on the tongues of mice to elicit immunization and after 3 immunizations at 4-week intervals, mice were challenged with a lethal dose of A/PR/8/34 to assess vaccine-induced protection. The 3 ODFVs containing 50, 250, or 750 μg of inactivated viruses elicited virus-specific antibody responses and virus neutralization in a dose-dependent manner. Dose-dependent antibody responses were also observed from the mucosal tissue samples, and also from antibody-secreting cells of the lungs and spleens. ODFV-induced cellular immunity, particularly germinal center B cells and T cells were also dose-dependent. Importantly, all 3 ODFVs evaluated in this study provided complete protection by strongly suppressing the pro-inflammatory cytokine production and lung virus titers. None of the immunized mice underwent noticeable weight loss nor succumbed to death, a phenomenon that was only observed in the infection challenge controls. These results indicated that the protection conferred by a low dose influenza vaccine formulated in ODF is comparable to that of a high-dose vaccine, thereby enabling vaccine dose sparing effect.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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