RNA Sequencing Protocols for Short-Read Sequencing.
| Autor: | Vasquez-Velez L; Genomics Center, Rutgers New Jersey Medical School, Newark, NJ, USA., D'Mello V; Genomics Center, Rutgers New Jersey Medical School, Newark, NJ, USA., Soteropoulos P; Department of Microbiology, Biochemistry, and Molecular Genetics, Rutgers New Jersey Medical School, Newark, NJ, USA. soteropa@njms.rutgers.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2866, pp. 125-158. |
| DOI: | 10.1007/978-1-0716-4192-7_8 |
| Abstrakt: | RNA sequencing (RNA-seq) methodologies allow the discovery of novel variants and transcripts. These comprise three general steps: (1) capture of RNA species of interest, (2) conversion of RNA to complementary DNA (cDNA), and (3) modification of cDNA to fit the sequencing platform. Here we describe four different library preparation protocols for short-read sequencing: cDNA synthesis with poly(A) selection, library preparation with ribosomal depletion, and cDNA synthesis with SMART ® (Switching Mechanism at 5' end of RNA Template) technology for low and Pico inputs. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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