Repurposing flubendazole for glioblastoma ferroptosis by affecting xCT and TFRC proteins.

Autor: Teng W; Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.; Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China., Ling Y; Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.; Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China., Long N; Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China., Cen W; Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China., Zhang H; Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China., Jiang L; Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China., Liu J; Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.; Department of Neurosurgery, Guizhou Provincial People's Hospital, Guiyang, Guizhou, China., Zhou X; Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.; Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China., Chu L; Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.; Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China.
Jazyk: angličtina
Zdroj: Journal of cellular and molecular medicine [J Cell Mol Med] 2024 Nov; Vol. 28 (22), pp. e70188.
DOI: 10.1111/jcmm.70188
Abstrakt: New uses of old drugs hold great promise for clinical translation. Flubendazole, an FDA-approved antiparasitic drug, has been shown to target p53 and promote apoptosis in glioblastoma (GBM) cells. However, its damaging mechanism in GBM remains elusive. Herein, we explored the ferroptosis-inducing ability of flubendazole on GBM cells. After treating glioma cell lines U251 and LN229 with the flubendazole (DMSO <1‰), cell viability was inhibited in a concentration-dependent manner (IC 50 for LN229 = 0.5331 μM, IC 50 for U251 = 0.6809 μM), attributed to the induction of ferroptosis, as evidenced by increased MDA levels, accumulation of ROS and lipid peroxides, change in mitochondrial membrane potential and structure. Protein analysis related to ferroptosis showed upregulation of TFRC, DMT1 and p53, alongside downregulation of xCT, FHC and GPX4 (p < 0.05). All-atom docking studies demonstrated that flubendazole bound closely with xCT, and TFRC, validating its role in inducing glioma ferroptosis via modulation of these proteins. Notably, flubendazole could damage the glioblastoma stem cells (GSC) that are typically resistant to other therapies, thereby possessing advantages in stopping glioma recurrence. This study delved into the mechanisms of flubendazole-induced ferroptosis in glioma, broadening its application and providing new ideas for new uses of other old drugs.
(© 2024 The Author(s). Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: