Impact of mixed Staphylococcus aureus-Pseudomonas aeruginosa biofilm on susceptibility to antimicrobial treatments in a 3D in vitro model.

Autor: Landa G; Aragon Institute of Nanoscience and Materials (INMA), CSIC-University of Zaragoza, Zaragoza, 50009, Spain. glanda@unizar.es.; Department of Chemical and Environmental Engineering, University of Zaragoza, Campus Río Ebro-Edificio I+D, C/ Poeta Mariano Esquillor S/N, Zaragoza, 50018, Spain. glanda@unizar.es.; Aragon Health Research Institute (IIS Aragon), Zaragoza, 50009, Spain. glanda@unizar.es., Clarhaut J; INSERM U1070 'Pharmacology of antimicrobial agents and resistances', University of Poitiers, Pôle Biologie Santé, 1 rue Georges Bonnet, Poitiers, 86022, France.; CHU Poitiers, 2 rue de la Milétrie, Poitiers, 86021, France., Buyck J; INSERM U1070 'Pharmacology of antimicrobial agents and resistances', University of Poitiers, Pôle Biologie Santé, 1 rue Georges Bonnet, Poitiers, 86022, France., Mendoza G; Aragon Health Research Institute (IIS Aragon), Zaragoza, 50009, Spain. gmendoza@iisaragon.es.; Department of Pharmacology and Physiology, Forensic and Legal Medicine, University of Zaragoza, Zaragoza, 50009, Spain. gmendoza@iisaragon.es., Arruebo M; Aragon Institute of Nanoscience and Materials (INMA), CSIC-University of Zaragoza, Zaragoza, 50009, Spain.; Department of Chemical and Environmental Engineering, University of Zaragoza, Campus Río Ebro-Edificio I+D, C/ Poeta Mariano Esquillor S/N, Zaragoza, 50018, Spain.; Aragon Health Research Institute (IIS Aragon), Zaragoza, 50009, Spain., Tewes F; INSERM U1070 'Pharmacology of antimicrobial agents and resistances', University of Poitiers, Pôle Biologie Santé, 1 rue Georges Bonnet, Poitiers, 86022, France.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 13; Vol. 14 (1), pp. 27877. Date of Electronic Publication: 2024 Nov 13.
DOI: 10.1038/s41598-024-79573-y
Abstrakt: Staphylococcus aureus and Pseudomonas aeruginosa are the most common bacteria co-isolated from chronic infected wounds. Their interactions remain unclear but this coexistence is beneficial for both bacteria and may lead to resistance to antimicrobial treatments. Besides, developing an in vitro model where this coexistence is recreated remains challenging, making difficult their study. The aim of this work was to develop a reliable polymicrobial in vitro model of both species to further understand their interrelationships and the effects of different antimicrobials in coculture. In this work, bioluminescent and fluorescent bacteria were used to evaluate the activity of two antiseptics (chlorhexidine and thymol) against these bacteria planktonically grown, or when forming single and mixed biofilms. At the doses tested (0.4-1,000 mg/L), thymol showed selective antimicrobial action against S. aureus in planktonic and biofilm states, in contrast with chlorhexidine which exerted antimicrobial effects against both bacteria. Furthermore, the initial conditions for both bacteria in the co-culture determined the antimicrobial outcome, showing that P. aeruginosa impaired the proliferation and metabolism of S. aureus. Moreover, S. aureus showed an increased tolerance against antiseptic treatments when co-cultured, attributed to the formation of a thicker mixed biofilm compared to those obtained when monocultured, and also, by the reduction of S. aureus metabolic activity induced by diffusible molecules produced by P. aeruginosa. This work underlines the relevance of polymicrobial populations and their crosstalk and microenvironment in the search of disruptive and effective treatments for polymicrobial biofilms.
Competing Interests: Declarations Competing interests The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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