Vaginal Mucosal Melanoma Cell Activation in Response to Photon or Carbon Ion Irradiation.

Autor:	Charalampopoulou A; Radiobiology Unit, CNAO National Center for Oncological Hadrontherapy, Pavia, Italy.; University School for Advanced Studies IUSS, Pavia, Italy., Barcellini A; Radiation Oncology Unit, Clinical Department, CNAO National Center for Oncological Hadrontherapy, Pavia, Italy.; Department of Internal Medicine and Therapeutics, University of Pavia, Pavia, Italy., Bistika M; Department of Biology and Biotechnology 'L.Spallanzani', Univeristy of Pavia, Pavia, Italy., Ivaldi GB; Radiation Oncology Unit, Istituti Clinici Scientifici Maugeri IRCCS, Pavia, Italy., Lillo S; Radiation Oncology Unit, Clinical Department, CNAO National Center for Oncological Hadrontherapy, Pavia, Italy., Magro G; Medical Physics Unit, Clinical Department, CNAO National Center for Oncological Hadrontherapy, Pavia, Italy., Orlandi E; Radiation Oncology Unit, Clinical Department, CNAO National Center for Oncological Hadrontherapy, Pavia, Italy.; Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy., Pullia MG; Research and Development Department, CNAO National Center for Oncological Hadrontherapy, Pavia, Italy., Ronchi S; Radiation Oncology Unit, Clinical Department, CNAO National Center for Oncological Hadrontherapy, Pavia, Italy., De Fatis PT; Medical Physics, Istituti Clinici Scientifici Maugeri IRCCS, Pavia, Italy., Facoetti A; Radiobiology Unit, CNAO National Center for Oncological Hadrontherapy, Pavia, Italy.
Jazyk:	angličtina
Zdroj:	International journal of particle therapy [Int J Part Ther] 2024 Sep 29; Vol. 14, pp. 100630. Date of Electronic Publication: 2024 Sep 29 (Print Publication: 2024).
DOI:	10.1016/j.ijpt.2024.100630
Abstrakt:	Purpose: Primary gynecological melanomas are uncommon with lower survival rates compared to cutaneous melanomas. Although melanocytes have been identified in a variety of mucosal membranes, little is known about their interactions or roles inside the mucosa layer. Melanin is a common pigment in nature and is endowed with several peculiar chemical, paramagnetic, and semiconductive characteristics. One of its latest explored functions is its interaction with ionizing radiation as a protective mechanism as well as its implication in the metastatic cascade of tumor cells. Materials and Methods: In this work, we analyzed in vitro the effects of different doses of photon and carbon ion irradiation on dendrite formation, pigmentation, migration, and invasion abilities of human mucosal melanoma cells of the vagina. We evaluated the morphology and melanin production of HMV-II cells exposed to photon and carbon ion beams with single doses between 0.5 and 10 Gy. Results: Our results showed that irradiation induces dendrite formation or elongation and pigmentation in HMV-II cells in a dose-type-dependent and radiation-type-dependent way but also a decrease in cell motility. Conclusion: The present study describes for the first time an induction of dendritic formation, melanin production, and alterations in migration and invasion abilities by low-linear energy transfer and high-linear energy transfer radiation in human mucosal melanoma cells, suggesting a radioprotective response to further possible exposures increasing the radioresistance of these cells. (© 2024 The Author(s).)
Databáze:	MEDLINE
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